SMMC-7721-CAS9 cell line

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SMMC-7721-CAS9 (Hela-contaminated cells, not for sales) Order

SMMC-7721-CAS9 cell line
Catalog#
YC-C056-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-B
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SMMC-7721-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
SMMC-7721-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the SMMC-7721 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a SMMC-7721-CAS9 cell line that can maintain long-term, stable and high expression.
Cutting Efficiency Validation
SMMC-7721-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the SMMC-7721-CAS9 stable cell pool which is electroporated by EIF2AK1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:SMMC-7721		 STR Info (Cell bank)
Cell Line:SMMC-7721
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 13.313.313.313.3
D7S820 12121212
D16S539 910910
VWA 161718.01618
TH01 7777
AMEL XXXX
TPOX 12121212
CSF1PO 10101010
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 180418). It is difficult to obtain early samples for testing because the cell line was established many years ago. However, it is clear that multiple samples are misidentified. The entry should be reassessed if early material can be found for testing.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
SMMC-7721-CAS9 cell line
SMMC-7721-CAS9
Catalog#
YC-C056-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

  • SMMC-7721 cell line

    Human Hepatoma Cell Line(SMMC-7721)
    Hela-contaminated cells, not for sales
      inquiry   More details

    Catalog#: YC-C056
    Size: 1*10^6
    Instruction: Human Hepatoma Cell Line(SMMC-7721)

Luciferase Stable Cell Lines

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Wild-type Cell Lines

Luciferase Stable Cell Lines

SMMC-7721-CAS9 (Hela-contaminated cells, not for sales) Order

SMMC-7721-CAS9 cell line
Catalog#
YC-C056-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-B
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SMMC-7721-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
SMMC-7721-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the SMMC-7721 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a SMMC-7721-CAS9 cell line that can maintain long-term, stable and high expression.
Cutting Efficiency Validation
SMMC-7721-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the SMMC-7721-CAS9 stable cell pool which is electroporated by EIF2AK1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:SMMC-7721		 STR Info (Cell bank)
Cell Line:SMMC-7721
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 13.313.313.313.3
D7S820 12121212
D16S539 910910
VWA 161718.01618
TH01 7777
AMEL XXXX
TPOX 12121212
CSF1PO 10101010
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 180418). It is difficult to obtain early samples for testing because the cell line was established many years ago. However, it is clear that multiple samples are misidentified. The entry should be reassessed if early material can be found for testing.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
SMMC-7721-CAS9 cell line
SMMC-7721-CAS9
Catalog#
YC-C056-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

  • SMMC-7721 cell line

    Human Hepatoma Cell Line(SMMC-7721)
    Hela-contaminated cells, not for sales
      inquiry   More details

    Catalog#: YC-C056
    Size: 1*10^6
    Instruction: Human Hepatoma Cell Line(SMMC-7721)

Luciferase Stable Cell Lines

Related Popular Products

Wild-type Cell Lines

Luciferase Stable Cell Lines


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